Tabriz University of Medical Sciences ID: 6620 Congress: 1st Tabriz International Life Science Conference and 12th Iran Biophysical Chemistry Conference Title: characterization and Cytotoxicity of Platinum Anticancer Complex Encapsulated in Beta-Casein-Chitosan Nanoparticles

Introduction: Clinical application of platinum-based anticancer drugs is largely limited by severe toxicity. Drug delivery systems are highly desired for improving the efficacy and applicability of these drugs. This study describes an alternative strategy for the delivery of a new synthesized Pt(II) complex (bipyridin morpholin dithio carbomate Pt(II) nitrate) by its encapsulating in the Beta-Casein (B-CN)-Chitosan(CS) nanoparticles. Beta-casein, an amphiphilic milk protein, self-associates into micelles in aqueous solutions. Methods: Initially, the influence of pH on the formation of colloidally stable nanocarrier system composed of Pt complex-loaded B-CN and chitosan noparticles was investigated using dynamic light scattering (DLS) and scanning electron microscopy (SEM). Then, the cytotoxicity of free and encapsulated Pt complex were evaluated on colorctal carcinoma HCT116 cells. Results: Formed Pt complex-loaded beta-casein-chitosan nanoparticles were stable and soluble at the pHs of 5.7 and 6.2. DLS data showed that the particles formed had sizes between 200 and 300 nm. At these pHs, the values of zeta-potential of nanoparticles were positive and formed particles were stable. SEM analysis confirmed the formation of nanoparticles with a good colloidal stability and an average particle size of 200 nm. Both cytotoxicity and cellular uptake of platinum were enhanced by its entrapping in B-CN-CS nanovehicles. Conclusions: These findings suggested that this novel drug-delivery system allows drugs to be thermodynamically stable in aqueous solutions and potentially useful for targeted drug-delivery applications. Beta-casein, Platinum complex, Chitosan, nanovehicles, drug delivery system Presentation: Poster